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Bacterial Infections of the Skin and Eyes

Learning Objectives

Despite the skin’s protective functions, infections are common. Gram-positive Staphylococcus spp. and Streptococcus spp. are responsible for many of the most common skin infections. However, many skin conditions are not strictly associated with a single pathogen. Opportunistic pathogens of many types may infect skin wounds, and individual cases with identical symptoms may result from different pathogens or combinations of pathogens.

In this section, we will examine some of the most important bacterial infections of the skin and eyes and discuss how biofilms can contribute to and exacerbate such infections. Key features of bacterial skin and eye infections are also summarized in the Disease Profile boxes throughout this section.

Staphylococcal Infections of the Skin

Staphylococcus species are commonly found on the skin, with S. epidermidis and S. hominis being prevalent in the normal microbiota. S. aureus is also commonly found in the nasal passages and on healthy skin, but pathogenic strains are often the cause of a broad range of infections of the skin and other body systems.

S. aureus is quite contagious. It is spread easily through skin-to-skin contact, and because many people are chronic nasal carriers (asymptomatic individuals who carry S. aureus in their nares), the bacteria can easily be transferred from the nose to the hands and then to fomites or other individuals. Because it is so contagious, S. aureus is prevalent in most community settings. This prevalence is particularly problematic in hospitals, where antibiotic-resistant strains of the bacteria may be present, and where immunocompromised patients may be more susceptible to infection. Resistant strains include methicillin-resistant S. aureus (MRSA), which can be acquired through health-care settings (hospital-acquired MRSA, or HA-MRSA) or in the community (community-acquired MRSA, or CA-MRSA). Hospital patients often arrive at health-care facilities already colonized with antibiotic-resistant strains of S. aureus that can be transferred to health-care providers and other patients. Some hospitals have attempted to detect these individuals in order to institute prophylactic measures, but they have had mixed success (see Eye on Ethics: Screening Patients for MRSA).

When a staphylococcal infection develops, choice of medication is important. As discussed above, many staphylococci (such as MRSA) are resistant to some or many antibiotics. Thus, antibiotic sensitivity is measured to identify the most suitable antibiotic. However, even before receiving the results of sensitivity analysis, suspected S. aureus infections are often initially treated with drugs known to be effective against MRSA, such as trimethoprim-sulfamethoxazole (TMP/SMZ), clindamycin, a tetracycline (doxycycline or minocycline), or linezolid.

The pathogenicity of staphylococcal infections is often enhanced by characteristic chemicals secreted by some strains. Staphylococcal virulence factors include hemolysins called staphylolysins, which are cytotoxic for many types of cells, including skin cells and white blood cells. Virulent strains of S. aureus are also coagulase-positive, meaning they produce coagulase, a plasma-clotting protein that is involved in abscess formation. They may also produce leukocidins, which kill white blood cells and can contribute to the production of pus and Protein A, which inhibits phagocytosis by binding to the constant region of antibodies. Some virulent strains of S. aureus also produce other toxins, such as toxic shock syndrome toxin-1 (see Virulence Factors of Bacterial and Viral Pathogens).

To confirm the causative agent of a suspected staphylococcal skin infection, samples from the wound are cultured. Under the microscope, gram-positive Staphylococcus species have cellular arrangements that form grapelike clusters; when grown on blood agar, colonies have a unique pigmentation ranging from opaque white to cream. A catalase test is used to distinguish Staphylococcus from Streptococcus, which is also a genus of gram-positive cocci and a common cause of skin infections. Staphylococcus species are catalase-positive while Streptococcus species are catalase-negative.

Other tests are performed on samples from the wound in order to distinguish coagulase-positive species of Staphylococcus (CoPS) such as S. aureus from common coagulase-negative species (CoNS) such as S. epidermidis. Although CoNS are less likely than CoPS to cause human disease, they can cause infections when they enter the body, as can sometimes occur via catheters, indwelling medical devices, and wounds. Passive agglutination testing can be used to distinguish CoPS from CoNS. If the sample is coagulase-positive, the sample is generally presumed to contain S. aureus. Additional genetic testing would be necessary to identify the particular strain of S. aureus.

Another way to distinguish CoPS from CoNS is by culturing the sample on mannitol salt agar (MSA). Staphylococcus species readily grow on this medium because they are tolerant of the high concentration of sodium chloride (7.5% NaCl). However, CoPS such as S. aureus ferment mannitol (which will be evident on a MSA plate), whereas CoNS such as S. epidermidis do not ferment mannitol but can be distinguished by the fermentation of other sugars such as lactose, malonate, and raffinose ([link]).

(a) A mannitol salt agar plate is used to distinguish different species of staphylococci. In this plate, S. aureus is on the left and S. epidermidis is in the right. Because S. aureus is capable of fermenting mannitol, it produces acids that cause the color to change to yellow. (b) This scanning electron micrograph shows the characteristic grapelike clusters of S. aureus. (credit a: modification of work by “ScienceProfOnline”/YouTube; credit b: modification of work by Centers for Disease Control and Prevention)
a) An agar plate with 2 regions of growth. One region has a yellow background, the other has a pink background. B) A micrograph of clusters of round cells. Each cell is just under 1 µm in diameter.
Screening Patients for MRSA

According to the CDC, 86% of invasive MRSA infections are associated in some way with healthcare, as opposed to being community-acquired. In hospitals and clinics, asymptomatic patients who harbor MRSA may spread the bacteria to individuals who are more susceptible to serious illness.

In an attempt to control the spread of MRSA, hospitals have tried screening patients for MRSA. If patients test positive following a nasal swab test, they can undergo decolonization using chlorhexidine washes or intranasal mupirocin. Some studies have reported substantial reductions in MRSA disease following implementation of these protocols, while others have not. This is partly because there is no standard protocol for these procedures. Several different MRSA identification tests may be used, some involving slower culturing techniques and others rapid testing. Other factors, such as the effectiveness of general hand-washing protocols, may also play a role in helping to prevent MRSA transmission. There are still other questions that need to be addressed: How frequently should patients be screened? Which individuals should be tested? From where on the body should samples be collected? Will increased resistance develop from the decolonization procedures?

Even if identification and decolonization procedures are perfected, ethical questions will remain. Should patients have the right to decline testing? Should a patient who tests positive for MRSA have the right to decline the decolonization procedure, and if so, should hospitals have the right to refuse treatment to the patient? How do we balance the individual’s right to receive care with the rights of other patients who could be exposed to disease as a result?

Superficial Staphylococcal Infections

S. aureus is often associated with pyoderma, skin infections that are purulent. Pus formation occurs because many strains of S. aureus produce leukocidins, which kill white blood cells. These purulent skin infections may initially manifest as folliculitis, but can lead to furuncles or deeper abscesses called carbuncles.

Folliculitis generally presents as bumps and pimples that may be itchy, red, and/or pus-filled. In some cases, folliculitis is self-limiting, but if it continues for more than a few days, worsens, or returns repeatedly, it may require medical treatment. Sweat, skin injuries, ingrown hairs, tight clothing, irritation from shaving, and skin conditions can all contribute to folliculitis. Avoidance of tight clothing and skin irritation can help to prevent infection, but topical antibiotics (and sometimes other treatments) may also help. Folliculitis can be identified by skin inspection; treatment is generally started without first culturing and identifying the causative agent.

In contrast, furuncles (boils) are deeper infections ([link]). They are most common in those individuals (especially young adults and teenagers) who play contact sports, share athletic equipment, have poor nutrition, live in close quarters, or have weakened immune systems. Good hygiene and skin care can often help to prevent furuncles from becoming more infective, and they generally resolve on their own. However, if furuncles spread, increase in number or size, or lead to systemic symptoms such as fever and chills, then medical care is needed. They may sometimes need to be drained (at which time the pathogens can be cultured) and treated with antibiotics.

When multiple boils develop into a deeper lesion, it is called a carbuncle ([link]). Because carbuncles are deeper, they are more commonly associated with systemic symptoms and a general feeling of illness. Larger, recurrent, or worsening carbuncles require medical treatment, as do those associated with signs of illness such as fever. Carbuncles generally need to be drained and treated with antibiotics. While carbuncles are relatively easy to identify visually, culturing and laboratory analysis of the wound may be recommended for some infections because antibiotic resistance is relatively common.

Proper hygiene is important to prevent these types of skin infections or to prevent the progression of existing infections.

Furuncles (boils) and carbuncles are infections of the skin often caused by Staphylococcus bacteria. (a) A furuncle contains pus and exhibits swelling. (b) A carbuncle is a pus-filled lesion that is typically deeper than the furuncle. It often forms from multiple furuncles. (credit a: modification of work by “Mahdouch”/Wikimedia Commons; credit b: modification of work by “Drvgaikwad”/Wikimedia Commons)
a) a photo of a small inflamed region with a white center. b) A large lesion with white and red.

Staphylococcal scalded skin syndrome (SSSS) is another superficial infection caused by S. aureus that is most commonly seen in young children, especially infants. Bacterial exotoxins first produce erythema (redness of the skin) and then severe peeling of the skin, as might occur after scalding ([link]). SSSS is diagnosed by examining characteristics of the skin (which may rub off easily), using blood tests to check for elevated white blood cell counts, culturing, and other methods. Intravenous antibiotics and fluid therapy are used as treatment.

A newborn with staphylococcal scalded skin syndrome (SSSS), which results in large regions of peeling, dead skin. (credit: modification of work by D Jeyakumari, R Gopal, M Eswaran, and C MaheshKumar)
Skin peeling off a hand.


The skin infection impetigo causes the formation of vesicles, pustules, and possibly bullae, often around the nose and mouth. Bullae are large, fluid-filled blisters that measure at least 5 mm in diameter. Impetigo can be diagnosed as either nonbullous or bullous. In nonbullous impetigo, vesicles and pustules rupture and become encrusted sores. Typically the crust is yellowish, often with exudate draining from the base of the lesion. In bullous impetigo, the bullae fill and rupture, resulting in larger, draining, encrusted lesions ([link]).

Especially common in children, impetigo is particularly concerning because it is highly contagious. Impetigo can be caused by S. aureus alone, by Streptococcus pyogenes alone, or by coinfection of S. aureus and S. pyogenes. Impetigo is often diagnosed through observation of its characteristic appearance, although culture and susceptibility testing may also be used.

Topical or oral antibiotic treatment is typically effective in treating most cases of impetigo. However, cases caused by S. pyogenes can lead to serious sequelae (pathological conditions resulting from infection, disease, injury, therapy, or other trauma) such as acute glomerulonephritis (AGN), which is severe inflammation in the kidneys.

Impetigo is characterized by vesicles, pustules, or bullae that rupture, producing encrusted sores. (credit: modification of work by FDA)
Red bumps on the upper region of the mouth.

Nosocomial S. epidermidis Infections

Though not as virulent as S. aureus, the staphylococcus S. epidermidis can cause serious opportunistic infections. Such infections usually occur only in hospital settings. S. epidermidis is usually a harmless resident of the normal skin microbiota. However, health-care workers can inadvertently transfer S. epidermidis to medical devices that are inserted into the body, such as catheters, prostheses, and indwelling medical devices. Once it has bypassed the skin barrier, S. epidermidis can cause infections inside the body that can be difficult to treat. Like S. aureus, S. epidermidis is resistant to many antibiotics, and localized infections can become systemic if not treated quickly. To reduce the risk of nosocomial (hospital-acquired) S. epidermidis, health-care workers must follow strict procedures for handling and sterilizing medical devices before and during surgical procedures.

  • Why are Staphylococcus aureus infections often purulent?

Streptococcal Infections of the Skin

Streptococcus are gram-positive cocci with a microscopic morphology that resembles chains of bacteria. Colonies are typically small (1–2 mm in diameter), translucent, entire edge, with a slightly raised elevation that can be either nonhemolytic, alpha-hemolytic, or beta-hemolytic when grown on blood agar ([link]). Additionally, they are facultative anaerobes that are catalase-negative.

Streptococcus pyogenes forms chains of cocci. (credit: modification of work by Centers for Disease Control and Prevention)
A micrograph of streptococcus pyogenes is shown.

The genus Streptococcus includes important pathogens that are categorized in serological Lancefield groups based on the distinguishing characteristics of their surface carbohydrates. The most clinically important streptococcal species in humans is S. pyogenes, also known as group A streptococcus (GAS). S. pyogenes produces a variety of extracellular enzymes, including streptolysins O and S, hyaluronidase, and streptokinase. These enzymes can aid in transmission and contribute to the inflammatory response.1 S. pyogenes also produces a capsule and M protein, a streptococcal cell wall protein. These virulence factors help the bacteria to avoid phagocytosis while provoking a substantial immune response that contributes to symptoms associated with streptococcal infections.

S. pyogenes causes a wide variety of diseases not only in the skin, but in other organ systems as well. Examples of diseases elsewhere in the body include pharyngitis and scarlet fever, which will be covered in later chapters.

Cellulitis, Erysipelas, and Erythema Nosodum

Common streptococcal conditions of the skin include cellulitis, erysipelas, and erythema nodosum. An infection that develops in the dermis or hypodermis can cause cellulitis, which presents as a reddened area of the skin that is warm to the touch and painful. The causative agent is often S. pyogenes, which may breach the epidermis through a cut or abrasion, although cellulitis may also be caused by staphylococci. S. pyogenes can also cause erysipelas, a condition that presents as a large, intensely inflamed patch of skin involving the dermis (often on the legs or face). These infections can be suppurative, which results in a bullous form of erysipelas. Streptococcal and other pathogens may also cause a condition called erythema nodosum, characterized by inflammation in the subcutaneous fat cells of the hypodermis. It sometimes results from a streptococcal infection, though other pathogens can also cause the condition. It is not suppurative, but leads to red nodules on the skin, most frequently on the shins ([link]).

In general, streptococcal infections are best treated through identification of the specific pathogen followed by treatment based upon that particular pathogen’s susceptibility to different antibiotics. Many immunological tests, including agglutination reactions and ELISAs, can be used to detect streptococci. Penicillin is commonly prescribed for treatment of cellulitis and erysipelas because resistance is not widespread in streptococci at this time. In most patients, erythema nodosum is self-limiting and is not treated with antimicrobial drugs. Recommended treatments may include nonsteroidal anti-inflammatory drugs (NSAIDs), cool wet compresses, elevation, and bed rest.

S. pyogenes can cause a variety of skin conditions once it breaches the skin barrier through a cut or wound. (a) Cellulitis presents as a painful, red rash. (b) Erysipelas presents as a raised rash, usually with clear borders. (c) Erythema nodosum is characterized by red lumps or nodules, typically on the lower legs. (credit a: modification of work by “Bassukas ID, Gaitanis G, Zioga A, Boboyianni C, Stergiopoulou C; credit b: modification of work by Centers for Disease Control and Prevention; credit c: modification of work by Dean C, Crow WT)
a) a red rash. B) swollen, red regions on the cheeks and nose. C) red lumps on the skin.

Necrotizing Fasciitis

Streptococcal infections that start in the skin can sometimes spread elsewhere, resulting in a rare but potentially life-threatening condition called necrotizing fasciitis, sometimes referred to as flesh-eating bacterial syndrome. S. pyogenes is one of several species that can cause this rare but potentially-fatal condition; others include Klebsiella, Clostridium, Escherichia coli, S. aureus, and Aeromonas hydrophila.

Necrotizing fasciitis occurs when the fascia, a thin layer of connective tissue between the skin and muscle, becomes infected. Severe invasive necrotizing fasciitis due to Streptococcus pyogenes occurs when virulence factors that are responsible for adhesion and invasion overcome host defenses. S. pyogenes invasins allow bacterial cells to adhere to tissues and establish infection. Bacterial proteases unique to S. pyogenes aggressively infiltrate and destroy host tissues, inactivate complement, and prevent neutrophil migration to the site of infection. The infection and resulting tissue death can spread very rapidly, as large areas of skin become detached and die. Treatment generally requires debridement (surgical removal of dead or infected tissue) or amputation of infected limbs to stop the spread of the infection; surgical treatment is supplemented with intravenous antibiotics and other therapies ([link]).

Necrotizing fasciitis does not always originate from a skin infection; in some cases there is no known portal of entry. Some studies have suggested that experiencing a blunt force trauma can increase the risk of developing streptococcal necrotizing fasciitis.2

(a) The left leg of this patient shows the clinical features of necrotizing fasciitis. (b) The same patient’s leg is surgically debrided to remove the infection. (credit a, b: modification of work by Piotr Smuszkiewicz, Iwona Trojanowska, and Hanna Tomczak)
a) skin with large black, grey and red regions. b) Cut skin in surgery.
  • How do staphylococcal infections differ in general presentation from streptococcal infections?
Part 2

Observing that Sam’s wound is purulent, the doctor tells him that he probably has a bacterial infection. She takes a sample from the lesion to send for laboratory analysis, but because it is Friday, she does not expect to receive the results until the following Monday. In the meantime, she prescribes an over-the-counter topical antibiotic ointment. She tells Sam to keep the wound clean and apply a new bandage with the ointment at least twice per day.

  • How would the lab technician determine if the infection is staphylococcal or streptococcal? Suggest several specific methods.
  • What tests might the lab perform to determine the best course of antibiotic treatment?

Jump to the next Clinical Focus box. Go back to the previous Clinical Focus box.

Pseudomonas Infections of the Skin

Another important skin pathogen is Pseudomonas aeruginosa, a gram-negative, oxidase-positive, aerobic bacillus that is commonly found in water and soil as well as on human skin. P. aeruginosa is a common cause of opportunistic infections of wounds and burns. It can also cause hot tub rash, a condition characterized by folliculitis that frequently afflicts users of pools and hot tubs (recall the Clinical Focus case in Microbial Biochemistry). P. aeruginosa is also the cause of otitis externa (swimmer’s ear), an infection of the ear canal that causes itching, redness, and discomfort, and can progress to fever, pain, and swelling ([link]).

(a) Hot tub folliculitis presents as an itchy red rash. It is typically caused by P. aeruginosa, a bacterium that thrives in wet, warm environments such as hot tubs. (b) Otitis externa (swimmer’s ear) may also be caused by P. aeruginosa or other bacteria commonly found in water. Inflammation of the outer ear and ear canal can lead to painful swelling. (credit b: modification of work by Klaus D. Peter)
a) photo of acne b) photo of swollen ear.

Wounds infected with P. aeruginosa have a distinctive odor resembling grape soda or fresh corn tortillas. This odor is caused by the 2-aminoacetophenone that is used by P. aeruginosa in quorum sensing and contributes to its pathogenicity. Wounds infected with certain strains of P. aeruginosa also produce a blue-green pus due to the pigments pyocyanin and pyoverdin, which also contribute to its virulence. Pyocyanin and pyoverdin are siderophores that help P. aeruginosa survive in low-iron environments by enhancing iron uptake. P. aeruginosa also produces several other virulence factors, including phospholipase C (a hemolysin capable of breaking down red blood cells), exoenzyme S (involved in adherence to epithelial cells), and exotoxin A (capable of causing tissue necrosis). Other virulence factors include a slime that allows the bacterium to avoid being phagocytized, fimbriae for adherence, and proteases that cause tissue damage. P. aeruginosa can be detected through the use of cetrimide agar, which is selective for Pseudomonas species ([link]).

(a) These P. aeruginosa colonies are growing on xylose lysine sodium deoxycholate (XLD) agar. (b) Pseudomonas spp. can produce a variety of blue-green pigments. (c) Pseudomonas spp. may produce fluorescein, which fluoresces green under ultraviolet light under the right conditions. (credit a: modification of work by Centers for Disease Control and Prevention)
a) A red plate with white colonies. B) A clear plate with green colonies; the green extends past the colony. C) a dark plate with glowing colonies.

Pseudomonas spp. tend to be resistant to most antibiotics. They often produce β-lactamases, may have mutations affecting porins (small cell wall channels) that affect antibiotic uptake, and may pump some antibiotics out of the cell, contributing to this resistance. Polymyxin B and gentamicin are effective, as are some fluoroquinolones. Otitis externa is typically treated with ear drops containing acetic acid, antibacterials, and/or steroids to reduce inflammation; ear drops may also include antifungals because fungi can sometimes cause or contribute to otitis externa. Wound infections caused by Pseudomonas spp. may be treated with topical antibiofilm agents that disrupt the formation of biofilms.


One of the most ubiquitous skin conditions is acne. Acne afflicts nearly 80% of teenagers and young adults, but it can be found in individuals of all ages. Higher incidence among adolescents is due to hormonal changes that can result in overproduction of sebum.

Acne occurs when hair follicles become clogged by shed skin cells and sebum, causing non-inflammatory lesions called comedones. Comedones (singular “comedo”) can take the form of whitehead and blackhead pimples. Whiteheads are covered by skin, whereas blackhead pimples are not; the black color occurs when lipids in the clogged follicle become exposed to the air and oxidize ([link]).

(a) Acne is characterized by whitehead and blackhead comedones that result from clogged hair follicles. (b) Blackheads, visible as black spots on the skin, have a dark appearance due to the oxidation of lipids in sebum via exposure to the air. (credit a: modification of work by Bruce Blaus)
a) diagram of blackhead formation. A normal pore in the skin becomes filled with material forming a whitehed. Darker material forms a blackhead. B) blackheads on a nose.

Often comedones lead to infection by Propionibacterium acnes, a gram-positive, non-spore-forming, aerotolerant anaerobic bacillus found on skin that consumes components of sebum. P. acnes secretes enzymes that damage the hair follicle, causing inflammatory lesions that may include papules, pustules, nodules, or pseudocysts, depending on their size and severity.

Treatment of acne depends on the severity of the case. There are multiple ways to grade acne severity, but three levels are usually considered based on the number of comedones, the number of inflammatory lesions, and the types of lesions. Mild acne is treated with topical agents that may include salicylic acid (which helps to remove old skin cells) or retinoids (which have multiple mechanisms, including the reduction of inflammation). Moderate acne may be treated with antibiotics (erythromycin, clindamycin), acne creams (e.g., benzoyl peroxide), and hormones. Severe acne may require treatment using strong medications such as isotretinoin (a retinoid that reduces oil buildup, among other effects, but that also has serious side effects such as photosensitivity). Other treatments, such as phototherapy and laser therapy to kill bacteria and possibly reduce oil production, are also sometimes used.


Sam uses the topical antibiotic over the weekend to treat his wound, but he does not see any improvement. On Monday, the doctor calls to inform him that the results from his laboratory tests are in. The tests show evidence of both Staphylococcus and Streptococcus in his wound. The bacterial species were confirmed using several tests. A passive agglutination test confirmed the presence of S. aureus. In this type of test, latex beads with antibodies cause agglutination when S. aureus is present. Streptococcus pyogenes was confirmed in the wound based on bacitracin (0.04 units) susceptibility as well as latex agglutination tests specific for S. pyogenes.

Because many strains of S. aureus are resistant to antibiotics, the doctor had also requested an antimicrobial susceptibility test (AST) at the same time the specimen was submitted for identification. The results of the AST indicated no drug resistance for the Streptococcus spp.; the Staphylococcus spp. showed resistance to several common antibiotics, but were susceptible to cefoxitin and oxacillin. Once Sam began to use these new antibiotics, the infection resolved within a week and the lesion healed.

Go back to the previous Clinical Focus box.


The zoonotic disease anthrax is caused by Bacillus anthracis, a gram-positive, endospore-forming, facultative anaerobe. Anthrax mainly affects animals such as sheep, goats, cattle, and deer, but can be found in humans as well. Sometimes called wool sorter’s disease, it is often transmitted to humans through contact with infected animals or animal products, such as wool or hides. However, exposure to B. anthracis can occur by other means, as the endospores are widespread in soils and can survive for long periods of time, sometimes for hundreds of years.

The vast majority of anthrax cases (95–99%) occur when anthrax endospores enter the body through abrasions of the skin.3 This form of the disease is called cutaneous anthrax. It is characterized by the formation of a nodule on the skin; the cells within the nodule die, forming a black eschar, a mass of dead skin tissue ([link]). The localized infection can eventually lead to bacteremia and septicemia. If untreated, cutaneous anthrax can cause death in 20% of patients.4 Once in the skin tissues, B. anthracis endospores germinate and produce a capsule, which prevents the bacteria from being phagocytized, and two binary exotoxins that cause edema and tissue damage. The first of the two exotoxins consists of a combination of protective antigen (PA) and an enzymatic lethal factor (LF), forming lethal toxin (LeTX). The second consists of protective antigen (PA) and an edema factor (EF), forming edema toxin (EdTX).

(a) Cutaneous anthrax is an infection of the skin by B. anthracis, which produces tissue-damaging exotoxins. Dead tissues accumulating in this nodule have produced a small black eschar. (b) Colonies of B. anthracis grown on sheep’s blood agar. (credit a, b: modification of work by Centers for Disease Control and Prevention)
a) A black nodule on skin. b) A red plate with grey colonies.

Less commonly, anthrax infections can be initiated through other portals of entry such as the digestive tract (gastrointestinal anthrax) or respiratory tract (pulmonary anthrax or inhalation anthrax). Typically, cases of noncutaneous anthrax are more difficult to treat than the cutaneous form. The mortality rate for gastrointestinal anthrax can be up to 40%, even with treatment. Inhalation anthrax, which occurs when anthrax spores are inhaled, initially causes influenza-like symptoms, but mortality rates are approximately 45% in treated individuals and 85% in those not treated. A relatively new form of the disease, injection anthrax, has been reported in Europe in intravenous drug users; it occurs when drugs are contaminated with B. anthracis. Patients with injection anthrax show signs and symptoms of severe soft tissue infection that differ clinically from cutaneous anthrax. This often delays diagnosis and treatment, and leads to a high mortality rate.5

B. anthracis colonies on blood agar have a rough texture and serrated edges that eventually form an undulating band ([link]). Broad spectrum antibiotics such as penicillin, erythromycin, and tetracycline are often effective treatments.

Unfortunately, B. anthracis has been used as a biological weapon and remains on the United Nations’ list of potential agents of bioterrorism.6 Over a period of several months in 2001, a number of letters were mailed to members of the news media and the United States Congress. As a result, 11 individuals developed cutaneous anthrax and another 11 developed inhalation anthrax. Those infected included recipients of the letters, postal workers, and two other individuals. Five of those infected with pulmonary anthrax died. The anthrax spores had been carefully prepared to aerosolize, showing that the perpetrator had a high level of expertise in microbiology.7

A vaccine is available to protect individuals from anthrax. However, unlike most routine vaccines, the current anthrax vaccine is unique in both its formulation and the protocols dictating who receives it.8 The vaccine is administered through five intramuscular injections over a period of 18 months, followed by annual boosters. The US Food and Drug Administration (FDA) has only approved administration of the vaccine prior to exposure for at-risk adults, such as individuals who work with anthrax in a laboratory, some individuals who handle animals or animal products (e.g., some veterinarians), and some members of the United States military. The vaccine protects against cutaneous and inhalation anthrax using cell-free filtrates of microaerophilic cultures of an avirulent, nonencapsulated strain of B. anthracis.9 The FDA has not approved the vaccine for routine use after exposure to anthrax, but if there were ever an anthrax emergency in the United States, patients could be given anthrax vaccine after exposure to help prevent disease.

Bacterial Infections of the Skin

Bacterial infections of the skin can cause a wide range of symptoms and syndromes, ranging from the superficial and relatively harmless to the severe and even fatal. Most bacterial skin infections can be diagnosed by culturing the bacteria and treated with antibiotics. Antimicrobial susceptibility testing is also often necessary because many strains of bacteria have developed antibiotic resistance. [link] summarizes the characteristics of some common bacterial skin infections.

Table titled: Bacterial Infections of the Skin. Columns: Disease, Pathogen, Signs and Symptoms, Transmission, and Antimicrobial Drugs. Acne, Propionibacterium acnes, Comedones (whiteheads, blackheads); papules, pustules, nodules, or pseudocysts, Not transmissible; clogged pores become infected by normal skin microbiota (P. acnes), Erythromycin, clindamycin. Anthrax (cutaneous), Bacillus anthracis, Eschar at site of infection; may lead to septicemia and can be fatal, Entry of B. anthracis endospores through cut or abrasion, Penicillin, erythromycin, or tetracycline. Cellulitis, Streptococcus pyogenes, Localized inflammation of dermis and hypodermis; skin red, warm, and painful to the touch, Entry of S. pyogenes through cut or abrasion, Oral or intravenous antibiotics (e.g., penicillin). Erysipelas, S. pyogenes, Inflamed, swollen patch of skin, often on face; may be suppurative, Entry of S. pyogenes through cut or abrasion, Oral or intravenous antibiotics (e.g., penicillin). Erythema nodosum, S. pyogenes, Small red nodules, often on shins, Associated with other streptococcal infection, None or anti-inflammatory drugs for severe cases. Impetigo, Staphylococcus aureus, S. pyogenes, Vesicles, pustules, and sometimes bullae around nose and mouth, Highly contagious, especially via contact Topical or oral antibiotics. Necrotizing fasciitis, S. pyogenes, Klebsiella, Clostridium, others, Infection of fascia and rapidly spreading tissue death; can lead to septic shock and death, Entry of bacteria through cut or abrasion, Intravenous broad-spectrum antibiotics. Otitis externa, Pseudomonas aeruginosa, Itching, redness, discomfort of ear canal, progressing to fever, pain, swelling, P. aeruginosa enters ear canal via pool or other water, Acidic ear drops with antibiotics, antifungals, steroids. Staphylococcal scalded skin syndrome (SSSS), S. aureus, Erythema and severe peeling of skin Infection of skin and mucous membranes, especially in children, Intravenous antibiotics, fluid therapy. Wound infections, P. aeruginosa, others, Formation of biofilm in or on wound. Exposure of wound to microbes in environment; poor wound hygiene, Polymyxin B, gentamicin, fluoroquinolones, topical anti-biofilm agents.

Bacterial Conjunctivitis

Like the skin, the surface of the eye comes in contact with the outside world and is somewhat prone to infection by bacteria in the environment. Bacterial conjunctivitis (pinkeye) is a condition characterized by inflammation of the conjunctiva, often accompanied by a discharge of sticky fluid (described as acute purulent conjunctivitis) ([link]). Conjunctivitis can affect one eye or both, and it usually does not affect vision permanently. Bacterial conjunctivitis is most commonly caused by Haemophilus influenzae, but can also be caused by other species such as Moraxella catarrhalis, S. pneumoniae, and S. aureus. The causative agent may be identified using bacterial cultures, Gram stain, and diagnostic biochemical, antigenic, or nucleic acid profile tests of the isolated pathogen. Bacterial conjunctivitis is very contagious, being transmitted via secretions from infected individuals, but it is also self-limiting. Bacterial conjunctivitis usually resolves in a few days, but topical antibiotics are sometimes prescribed. Because this condition is so contagious, medical attention is recommended whenever it is suspected. Individuals who use contact lenses should discontinue their use when conjunctivitis is suspected. Certain symptoms, such as blurred vision, eye pain, and light sensitivity, can be associated with serious conditions and require medical attention.

Acute, purulent, bacterial conjunctivitis causes swelling and redness in the conjunctiva, the membrane lining the whites of the eyes and the inner eyelids. It is often accompanied by a yellow, green, or white discharge, which can dry and become encrusted on the eyelashes. (credit: “Tanalai”/Wikimedia Commons)
Eye with yellow discharge.

Neonatal Conjunctivitis

Newborns whose mothers have certain sexually transmitted infections are at risk of contracting ophthalmia neonatorum or inclusion conjunctivitis, which are two forms of neonatal conjunctivitis contracted through exposure to pathogens during passage through the birth canal. Gonococcal ophthalmia neonatorum is caused by Neisseria gonorrhoeae, the bacterium that causes the STD gonorrhea ([link]). Inclusion (chlamydial) conjunctivitis is caused by Chlamydia trachomatis, the anaerobic, obligate, intracellular parasite that causes the STD chlamydia.

To prevent gonoccocal ophthalmia neonatorum, silver nitrate ointments were once routinely applied to all infants’ eyes shortly after birth; however, it is now more common to apply antibacterial creams or drops, such as erythromycin. Most hospitals are required by law to provide this preventative treatment to all infants, because conjunctivitis caused by N. gonorrhoeae, C. trachomatis, or other bacteria acquired during a vaginal delivery can have serious complications. If untreated, the infection can spread to the cornea, resulting in ulceration or perforation that can cause vision loss or even permanent blindness. As such, neonatal conjunctivitis is treated aggressively with oral or intravenous antibiotics to stop the spread of the infection. Causative agents of inclusion conjunctivitis may be identified using bacterial cultures, Gram stain, and diagnostic biochemical, antigenic, or nucleic acid profile tests.

A newborn suffering from gonoccocal opthalmia neonatorum. Left untreated, purulent discharge can scar the cornea, causing loss of vision or permanent blindness. (credit: Centers for Disease Control and Prevention)
Swollen eyes with discharge.
  • Compare and contrast bacterial conjunctivitis with neonatal conjunctivitis.


Trachoma, or granular conjunctivitis, is a common cause of preventable blindness that is rare in the United States but widespread in developing countries, especially in Africa and Asia. The condition is caused by the same species that causes neonatal inclusion conjunctivitis in infants, Chlamydia trachomatis. C. trachomatis can be transmitted easily through fomites such as contaminated towels, bed linens, and clothing and also by direct contact with infected individuals. C. trachomatis can also be spread by flies that transfer infected mucous containing C. trachomatis from one human to another.

Infection by C. trachomatis causes chronic conjunctivitis, which leads to the formation of necrotic follicles and scarring in the upper eyelid. The scars turn the eyelashes inward (a condition known as trichiasis) and mechanical abrasion of the cornea leads to blindness ([link]). Antibiotics such as azithromycin are effective in treating trachoma, and outcomes are good when the disease is treated promptly. In areas where this disease is common, large public health efforts are focused on reducing transmission by teaching people how to avoid the risks of the infection.

(a) If trachoma is not treated early with antibiotics, scarring on the eyelid can lead to trichiasis, a condition in which the eyelashes turn inward. (b) Trichiasis leads to blindness if not corrected by surgery, as shown here. (credit b: modification of work by Otis Historical Archives National Museum of Health & Medicine)
a) eye with turned in eyelids. B) photo of eye surgery.
SAFE Eradication of Trachoma

Though uncommon in the United States and other developed nations, trachoma is the leading cause of preventable blindness worldwide, with more than 4 million people at immediate risk of blindness from trichiasis. The vast majority of those affected by trachoma live in Africa and the Middle East in isolated rural or desert communities with limited access to clean water and sanitation. These conditions provide an environment conducive to the growth and spread of Chlamydia trachomatis, the bacterium that causes trachoma, via wastewater and eye-seeking flies.

In response to this crisis, recent years have seen major public health efforts aimed at treating and preventing trachoma. The Alliance for Global Elimination of Trachoma by 2020 (GET 2020), coordinated by the World Health Organization (WHO), promotes an initiative dubbed “SAFE,” which stands for “Surgery, Antibiotics, Facial cleanliness, and Environmental improvement.” The Carter Center, a charitable, nongovernment organization led by former US President Jimmy Carter, has partnered with the WHO to promote the SAFE initiative in six of the most critically impacted nations in Africa. Through its Trachoma Control Program, the Carter Center trains and equips local surgeons to correct trichiasis and distributes antibiotics to treat trachoma. The program also promotes better personal hygiene through health education and improves sanitation by funding the construction of household latrines. This reduces the prevalence of open sewage, which provides breeding grounds for the flies that spread trachoma.

Bacterial Keratitis

Keratitis can have many causes, but bacterial keratitis is most frequently caused by Staphylococcus epidermidis and/or Pseudomonas aeruginosa. Contact lens users are particularly at risk for such an infection because S. epidermidis and P. aeruginosa both adhere well to the surface of the lenses. Risk of infection can be greatly reduced by proper care of contact lenses and avoiding wearing lenses overnight. Because the infection can quickly lead to blindness, prompt and aggressive treatment with antibiotics is important. The causative agent may be identified using bacterial cultures, Gram stain, and diagnostic biochemical, antigenic, or nucleic acid profile tests of the isolated pathogen.

Biofilms and Infections of the Skin and Eyes

When treating bacterial infections of the skin and eyes, it is important to consider that few such infections can be attributed to a single pathogen. While biofilms may develop in other parts of the body, they are especially relevant to skin infections (such as those caused by S. aureus or P. aeruginosa) because of their prevalence in chronic skin wounds. Biofilms develop when bacteria (and sometimes fungi) attach to a surface and produce extracellular polymeric substances (EPS) in which cells of multiple organisms may be embedded. When a biofilm develops on a wound, it may interfere with the natural healing process as well as diagnosis and treatment.

Because biofilms vary in composition and are difficult to replicate in the lab, they are still not thoroughly understood. The extracellular matrix of a biofilm consists of polymers such as polysaccharides, extracellular DNA, proteins, and lipids, but the exact makeup varies. The organisms living within the extracellular matrix may include familiar pathogens as well as other bacteria that do not grow well in cultures (such as numerous obligate anaerobes). This presents challenges when culturing samples from infections that involve a biofilm. Because only some species grow in vitro, the culture may contain only a subset of the bacterial species involved in the infection.

Biofilms confer many advantages to the resident bacteria. For example, biofilms can facilitate attachment to surfaces on or in the host organism (such as wounds), inhibit phagocytosis, prevent the invasion of neutrophils, and sequester host antibodies. Additionally, biofilms can provide a level of antibiotic resistance not found in the isolated cells and colonies that are typical of laboratory cultures. The extracellular matrix provides a physical barrier to antibiotics, shielding the target cells from exposure. Moreover, cells within a biofilm may differentiate to create subpopulations of dormant cells called persister cells. Nutrient limitations deep within a biofilm add another level of resistance, as stress responses can slow metabolism and increase drug resistance.

Bacterial Infections of the Eyes

A number of bacteria are able to cause infection when introduced to the mucosa of the eye. In general, bacterial eye infections can lead to inflammation, irritation, and discharge, but they vary in severity. Some are typically short-lived, and others can become chronic and lead to permanent eye damage. Prevention requires limiting exposure to contagious pathogens. When infections do occur, prompt treatment with antibiotics can often limit or prevent permanent damage. [link] summarizes the characteristics of some common bacterial infections of the eyes.

Table titled: Bacterial Infections of the Eyes. Columns: Disease, Pathogen, Signs and Symptoms, Transmission, and Antimicrobial Drugs. Acute bacterial conjunctivitis, Haemophilus influenza, Inflammation of conjunctiva with purulent discharge, Exposure to secretions from infected individuals, Broad-spectrum topical antibiotics. Bacterial keratitis, Staphylococcus epidermidis, Pseudomonas aeruginosa, Redness and irritation of eye, blurred vision, sensitivity to light; progressive corneal scarring, which can lead to blindness, Exposure to pathogens on contaminated contact lenses, Antibiotic eye drops (e.g., with fluoroquinolones). Neonatal conjunctivitis Chlamydia trachomatis, Neisseria gonorrhoeae, Inflammation of conjunctiva, purulent discharge, scarring and perforation of cornea; may lead to blindness, Neonate exposed to pathogens in birth canal of mother with chlamydia or gonorrhea Erythromycin. Trachoma (granular conjunctivitis), C. trachomatis, Chronic conjunctivitis, trichiasis, scarring, blindness, Contact with infected individuals or contaminated fomites; transmission by eye-seeking flies, Azithromycin.

Key Concepts and Summary

Multiple Choice

Staphylococcus aureus is most often associated with being

  1. coagulase-positive.
  2. coagulase-negative.
  3. catalase-negative.
  4. gram-negative


M protein is produced by

  1. Pseudomonas aeruginosa
  2. Staphylococcus aureus
  3. Propionibacterium acnes
  4. Streptococcus pyogenes


___________ is a major cause of preventable blindness that can be reduced through improved sanitation.

  1. Ophthalmia neonatorum
  2. Keratitis
  3. Trachoma
  4. Cutaneous anthrax


Which species is frequently associated with nosocomial infections transmitted via medical devices inserted into the body?

  1. Staphylococcus epidermidis
  2. Streptococcus pyogenes
  3. Proproniobacterium acnes
  4. Bacillus anthracis


Fill in the Blank

A purulent wound produces ________.


Short Answer

How are leukocidins associated with pus production?

What is a good first test to distinguish streptococcal infections from staphylococcal infections?


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  2. 2 Nuwayhid, Z.B., Aronoff, D.M., and Mulla, Z.D.. “Blunt Trauma as a Risk Factor for Group A Streptococcal Necrotizing Fasciitis.” Annals of Epidemiology (2007) 17:878–881.
  3. 3 Shadomy, S.V., Traxler, R.M., and Marston, C.K. “Infectious Diseases Related to Travel: Anthrax” 2015. Centers for Disease Control and Prevention. http://wwwnc.cdc.gov/travel/yellowbook/2016/infectious-diseases-related-to-travel/anthrax. Accessed Sept 14, 2016.
  4. 4 US FDA. “Anthrax.” 2015. http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ucm061751.htm. Accessed Sept 14, 2016.
  5. 5 Berger, T., Kassirer, M., and Aran, A.A.. “Injectional Anthrax—New Presentation of an Old Disease.” Euro Surveillance 19 (2014) 32. http://www.ncbi.nlm.nih.gov/pubmed/25139073. Accessed Sept 14, 2016.
  6. 6 United Nations Office at Geneva. “What Are Biological and Toxin Weapons?” http://www.unog.ch/80256EE600585943/%28httpPages%29/29B727532FECBE96C12571860035A6DB?. Accessed Sept 14, 2016.
  7. 7 Federal Bureau of Investigation. “Famous Cases and Criminals: Amerithrax or Anthrax Investigation.” https://www.fbi.gov/history/famous-cases/amerithrax-or-anthrax-investigation. Accessed Sept 14, 2016.
  8. 8 Centers for Disease Control and Prevention. “Anthrax: Medical Care: Prevention: Antibiotics.” http://www.cdc.gov/anthrax/medical-care/prevention.html. Accessed Sept 14, 2016.
  9. 9 Emergent Biosolutions. AVA (BioThrax) vaccine package insert (Draft). Nov 2015. http://www.fda.gov/downloads/biologicsbloodvaccines/bloodbloodproducts/approvedproducts/licensedproductsblas/ucm074923.pdf.

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