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Learning Objectives

Describe the chemical composition of lipids

Although they are composed primarily of carbon and hydrogen, lipid molecules may also contain oxygen, nitrogen, sulfur, and phosphorous. Lipids serve numerous and diverse purposes in the structure and functions of organisms. They can be a source of nutrients, a storage form for carbon, energy-storage molecules, or structural components of membranes and hormones. Lipids comprise a broad class of many chemically distinct compounds, the most common of which are discussed in this section.

Fatty Acids and Triacylglycerides

The fatty acids are lipids that contain long-chain hydrocarbons terminated with a carboxylic acid functional group. Because the long hydrocarbon chain, fatty acids are hydrophobic (“water fearing”) or nonpolar. Fatty acids with hydrocarbon chains that contain only single bonds are called saturated fatty acids because they have the greatest number of hydrogen atoms possible and are, therefore, “saturated” with hydrogen. Fatty acids with hydrocarbon chains containing at least one double bond are called unsaturated fatty acids because they have fewer hydrogen atoms. Saturated fatty acids have a straight, flexible carbon backbone, whereas unsaturated fatty acids have “kinks” in their carbon skeleton because each double bond causes a rigid bend of the carbon skeleton. These differences in saturated versus unsaturated fatty acid structure result in different properties for the corresponding lipids in which the fatty acids are incorporated. For example, lipids containing saturated fatty acids are solids at room temperature, whereas lipids containing unsaturated fatty acids are liquids.

A triacylglycerol, or triglyceride, is formed when three fatty acids are chemically linked to a glycerol molecule ([link]). Triglycerides are the primary components of adipose tissue (body fat), and are major constituents of sebum (skin oils). They play an important metabolic role, serving as efficient energy-storage molecules that can provide more than double the caloric content of both carbohydrates and proteins.

Triglycerides are composed of a glycerol molecule attached to three fatty acids by a dehydration synthesis reaction.
A diagram showing a triglyceride is made of a glycerol and three fatty acids. Glycerol is a 3 carbon chain with an OH on each carbon. The H on each OH is highlighted. Fatty acids are long carbon chains with a C that has an OH and a double bonded O at the end. The OH of this C is highlighted. Three fatty acids are shown. Each fatty acid binds to one of the O’s from the OH groups on each Carbon on glycerol. The result is a triglyceride (or neutral fat) and 3 water molecules.

phospholipids and Biological Membranes

Triglycerides are classified as simple lipids because they are formed from just two types of compounds: glycerol and fatty acids. In contrast, complex lipids contain at least one additional component, for example, a phosphate group (phospholipids) or a carbohydrate moiety (glycolipids). [link] depicts a typical phospholipid composed of two fatty acids linked to glycerol (a diglyceride). The two fatty acid carbon chains may be both saturated, both unsaturated, or one of each. Instead of another fatty acid molecule (as for triglycerides), the third binding position on the glycerol molecule is occupied by a modified phosphate group.

This illustration shows a phospholipid with two different fatty acids, one saturated and one unsaturated, bonded to the glycerol molecule. The unsaturated fatty acid has a slight kink in its structure due to the double bond.
A drawing of a phospholipid as a large circle with 2 rectangles projecting from the bottom. The circle is labeled hydrophilic head and contains glycerol (which contains 3 carbons). Attached ot one of these carbons is a phosphate (which is a phosphorus attached to 4 oxygen atoms). The rectangles at the bottom are both long carbon chains labeled as hydrophobic tails. One of the chains is a straight zig-zag line and is labeled saturated fatty acid. The other has a double bond that creates a bend in the line; this is labeled unsaturated fatty acid.

The molecular structure of lipids results in unique behavior in aqueous environments. [link] depicts the structure of a triglyceride. Because all three substituents on the glycerol backbone are long hydrocarbon chains, these compounds are nonpolar and not significantly attracted to polar water molecules—they are hydrophobic. Conversely, phospholipids such as the one shown in [link] have a negatively charged phosphate group. Because the phosphate is charged, it is capable of strong attraction to water molecules and thus is hydrophilic, or “water loving.” The hydrophilic portion of the phospholipid is often referred to as a polar “head,” and the long hydrocarbon chains as nonpolar “tails.” A molecule presenting a hydrophobic portion and a hydrophilic moiety is said to be amphipathic. Notice the “R” designation within the hydrophilic head depicted in [link], indicating that a polar head group can be more complex than a simple phosphate moiety. Glycolipids are examples in which carbohydrates are bonded to the lipids’ head groups.

The amphipathic nature of phospholipids enables them to form uniquely functional structures in aqueous environments. As mentioned, the polar heads of these molecules are strongly attracted to water molecules, and the nonpolar tails are not. Because of their considerable lengths, these tails are, in fact, strongly attracted to one another. As a result, energetically stable, large-scale assemblies of phospholipid molecules are formed in which the hydrophobic tails congregate within enclosed regions, shielded from contact with water by the polar heads ([link]). The simplest of these structures are micelles, spherical assemblies containing a hydrophobic interior of phospholipid tails and an outer surface of polar head groups. Larger and more complex structures are created from lipid-bilayer sheets, or unit membranes, which are large, two-dimensional assemblies of phospholipids congregated tail to tail. The cell membranes of nearly all organisms are made from lipid-bilayer sheets, as are the membranes of many intracellular components. These sheets may also form lipid-bilayer spheres that are the structural basis of vesicles and liposomes, subcellular components that play a role in numerous physiological functions.

phospholipids tend to arrange themselves in aqueous solution forming liposomes, micelles, or lipid bilayer sheets. (credit: modification of work by Mariana Ruiz Villarreal)
A lipid bilayer sheet is when there are 2 rows of phospholipids across each other forming a flat surface. The polar heads of all phospholipids are towards the outside of the sheet, and the nonpolar tails are towards the inside. This lipid-bilyaer can also form a sphere. The lipid-bilayer forms the surface of the sphere; the  polar heads are on the outside of the sphere and lining the inside space of the sphere.  Lipids can also form a single-layer sphere where the outside of the sphere is the polar heads and the nonpolar tails fill the center of the sphere.

Isoprenoids and Sterols

The isoprenoids are branched lipids, also referred to as terpenoids, that are formed by chemical modifications of the isoprene molecule ([link]). These lipids play a wide variety of physiological roles in plants and animals, with many technological uses as pharmaceuticals (capsaicin), pigments (e.g., orange beta carotene, xanthophylls), and fragrances (e.g., menthol, camphor, limonene [lemon fragrance], and pinene [pine fragrance]). Long-chain isoprenoids are also found in hydrophobic oils and waxes. Waxes are typically water resistant and hard at room temperature, but they soften when heated and liquefy if warmed adequately. In humans, the main wax production occurs within the sebaceous glands of hair follicles in the skin, resulting in a secreted material called sebum, which consists mainly of triacylglycerol, wax esters, and the hydrocarbon squalene. There are many bacteria in the microbiota on the skin that feed on these lipids. One of the most prominent bacteria that feed on lipids is Propionibacterium acnes, which uses the skin’s lipids to generate short-chain fatty acids and is involved in the production of acne.

Five-carbon isoprene molecules are chemically modified in various ways to yield isoprenoids.
Alpha-pinene is a carbon ring with added carbon projections. Camphor is a carbon ring with added carbon projections and a double bonded oxygen on one carbon. Isophrene is a 4 carbon chain with another carbon attached to carbon 2. Limonene is a carbon ring with a carbon attached to on one end and another carbon attached to the other end; this carbon has 2 carbons attached to it. Menthol i s a carbon ring with a carbon attached to on one end and another carbon attached to the other end; this carbon has 2 carbons attached to it. One more carbon corner has an OH group. Beta-carotene is two carbon rings attached by a long carbon chain.

Another type of lipids are steroids, complex, ringed structures that are found in cell membranes; some function as hormones. The most common types of steroids are sterols, which are steroids containing an OH group. These are mainly hydrophobic molecules, but also have hydrophilic hydroxyl groups. The most common sterol found in animal tissues is cholesterol. Its structure consists of four rings with a double bond in one of the rings, and a hydroxyl group at the sterol-defining position. The function of cholesterol is to strengthen cell membranes in eukaryotes and in bacteria without cell walls, such as Mycoplasma. Prokaryotes generally do not produce cholesterol, although bacteria produce similar compounds called hopanoids, which are also multiringed structures that strengthen bacterial membranes ([link]). Fungi and some protozoa produce a similar compound called ergosterol, which strengthens the cell membranes of these organisms.

Cholesterol and hopene (a hopanoid compound) are molecules that reinforce the structure of the cell membranes in eukaryotes and prokaryotes, respectively.
Cholesterol is made of 3 hexagons attached along their edges. The third hexagon has a pentagon attached along an edge. The pentagon has a carbon chain attached to it. Hopene is made of 4 hexagons attached along their edges. The last hexagon has a pentagon. The pentagon has a short carbon chain.
Part 2

The moisturizing cream prescribed by Penny’s doctor was a topical corticosteroid cream containing hydrocortisone. Hydrocortisone is a synthetic form of cortisol, a corticosteroid hormone produced in the adrenal glands, from cholesterol. When applied directly to the skin, it can reduce inflammation and temporarily relieve minor skin irritations, itching, and rashes by reducing the secretion of histamine, a compound produced by cells of the immune system in response to the presence of pathogens or other foreign substances. Because histamine triggers the body’s inflammatory response, the ability of hydrocortisone to reduce the local production of histamine in the skin effectively suppresses the immune system and helps limit inflammation and accompanying symptoms such as pruritus (itching) and rashes.

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Key Concepts and Summary

Multiple Choice

Which of the following describes lipids?

  1. a source of nutrients for organisms
  2. energy-storage molecules
  3. molecules having structural role in membranes
  4. molecules that are part of hormones and pigments
  5. all of the above


Molecules bearing both polar and nonpolar groups are said to be which of the following?

  1. hydrophilic
  2. amphipathic
  3. hydrophobic
  4. polyfunctional



Lipids are a naturally occurring group of substances that are not soluble in water but are freely soluble in organic solvents.


Fatty acids having no double bonds are called “unsaturated.”


A triglyceride is formed by joining three glycerol molecules to a fatty acid backbone in a dehydration reaction.


Fill in the Blank

Waxes contain esters formed from long-chain __________ and saturated __________, and they may also contain substituted hydrocarbons.

alcohols; fatty acids

Cholesterol is the most common member of the __________ group, found in animal tissues; it has a tetracyclic carbon ring system with a __________ bond in one of the rings and one free __________group.

steroid; double; hydroxyl

Critical Thinking

Microorganisms can thrive under many different conditions, including high-temperature environments such as hot springs. To function properly, cell membranes have to be in a fluid state. How do you expect the fatty acid content (saturated versus unsaturated) of bacteria living in high-temperature environments might compare with that of bacteria living in more moderate temperatures?

Short Answer

Describe the structure of a typical phospholipid. Are these molecules polar or nonpolar?

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